Ontology highlight
ABSTRACT:
SUBMITTER: Zhang Q
PROVIDER: S-EPMC5788022 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Zhang Qiang Q Huang Hai H Zhang Luqing L Wu Roland R Chung Chan-I CI Zhang Shao-Qing SQ Torra Joaquim J Schepis Antonino A Coughlin Shaun R SR Kornberg Thomas B TB Shu Xiaokun X
Molecular cell 20180104 2
Visualizing dynamics of kinase activity in living animals is essential for mechanistic understanding of cell and developmental biology. We describe GFP-based kinase reporters that phase-separate upon kinase activation via multivalent protein-protein interactions, forming intensively fluorescent droplets. Called SPARK (separation of phases-based activity reporter of kinase), these reporters have large dynamic range (fluorescence change), high brightness, fast kinetics, and are reversible. The SPA ...[more]