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Deciphering the Pharmacological Properties of Methanol Extract of Psychotria calocarpa Leaves by In Vivo, In Vitro and In Silico Approaches.

ABSTRACT: The present study explores the neuropharmacological, antinociceptive, antidiarrheal, antioxidant, thrombolytic and cytotoxic activity of methanol extract of Psychotria calocarpa leaves (MEPC). In anxiolytic activity testing of MEPC by elevated plus maze test, hole-board test and light-dark test, the extract exhibited a dose-dependent reduction of anxiety while the open field test observed a decreased locomotion. The administration of MEPC revealed a significant dose-dependent reduction of depressant behavior in forced swimming and tail suspension test. Additionally, the antinociceptive and antidiarrheal activity exposed a significant reduction of nociception and diarrheal behavior at the highest dose. In addition, a strong antioxidant activity was observed in DPPH-free radical-scavenging assay (IC50 = 461.05 ?g/mL), total phenol content (118.31 ± 1.12 mg) and total flavonoid content (100.85 ± 0.97 mg). The significant clot-lysis activity was also observed with moderate toxicity (LC50 = 247.92 ?g/mL) level in the lethality assay of brine shrimp. Moreover, in silico molecular docking study showed that the compound Psychotriasine could offer promising active site interactions for binding proteins. Furthermore, ADME/T and toxicological properties of the compound satisfied the Lipinski's rule of five and Veber rules for drug-like potential and toxicity level. Overall, MEPC had a potential neuropharmacological, antinociceptive, antidiarrheal and antioxidant activity that warranted further investigation.

SUBMITTER: Bristy TA

PROVIDER: S-EPMC7463710 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Deciphering the Pharmacological Properties of Methanol Extract of <i>Psychotria calocarpa</i> Leaves by In Vivo, In Vitro and In Silico Approaches.

Bristy Tahmina Akter TA Barua Niloy N Montakim Tareq Abu A Sakib Shahenur Alam SA Etu Saida Tasnim ST Chowdhury Kamrul Hasan KH Jyoti Mifta Ahmed MA Aziz Md Arfin Ibn MA Reza A S M Ali ASMA Caiazzo Elisabetta E Romano Barbara B Tareq Syed Mohammed SM Tareq Syed Mohammed SM Emran Talha Bin TB Capasso Raffaele R

Pharmaceuticals (Basel, Switzerland) 20200806 8

The present study explores the neuropharmacological, antinociceptive, antidiarrheal, antioxidant, thrombolytic and cytotoxic activity of methanol extract of <i>Psychotria calocarpa</i> leaves (MEPC). In anxiolytic activity testing of MEPC by elevated plus maze test, hole-board test and light-dark test, the extract exhibited a dose-dependent reduction of anxiety while the open field test observed a decreased locomotion. The administration of MEPC revealed a significant dose-dependent reduction of ...[more]

PMID: 32781707

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Dataset Information

Deciphering the Pharmacological Properties of Methanol Extract of Psychotria calocarpa Leaves by In Vivo, In Vitro and In Silico Approaches.

Publications

Deciphering the Pharmacological Properties of Methanol Extract of <i>Psychotria calocarpa</i> Leaves by In Vivo, In Vitro and In Silico Approaches.

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