Ontology highlight
ABSTRACT:
SUBMITTER: Bezalel-Buch R
PROVIDER: S-EPMC7470978 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Bezalel-Buch Rachel R Cheun Young K YK Roy Upasana U Schärer Orlando D OD Burgers Peter M PM
Nucleic acids research 20200901 15
DNA polymerase ζ (Pol ζ) and Rev1 are essential for the repair of DNA interstrand crosslink (ICL) damage. We have used yeast DNA polymerases η, ζ and Rev1 to study translesion synthesis (TLS) past a nitrogen mustard-based interstrand crosslink (ICL) with an 8-atom linker between the crosslinked bases. The Rev1-Pol ζ complex was most efficient in complete bypass synthesis, by 2-3 fold, compared to Pol ζ alone or Pol η. Rev1 protein, but not its catalytic activity, was required for efficient TLS. ...[more]