Identification of microRNA-451a as a Novel Circulating Biomarker for Colorectal Cancer Diagnosis.

ABSTRACT: Background:Colorectal cancer (CRC) is one of the leading causes of cancer death worldwide. Successful treatment of CRC relies on accurate early diagnosis, which is currently a challenge due to its complexity and personalized pathologies. Thus, novel molecular biomarkers are needed for early CRC detection. Methods:Gene and microRNA microarray profiling of CRC tissues and miRNA-seq data were analyzed. Candidate microRNA biomarkers were predicted using both CRC-specific network and miRNA-BD tool. Validation analyses were carried out to interrogate the identified candidate CRC biomarkers. Results:We identified miR-451a as a potential early CRC biomarker circulating in patient's serum. The dysregulation of miR-451a was revealed both in primary tumors and in patients' sera. Downstream analysis validated the tumor suppressor role of miR-451a and high sensitivity of miR-451a in CRC patients, further confirming its potential role as CRC circulation biomarker. Conclusion:The miR-451a is a potential circulating biomarker for early CRC diagnosis.

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC7474364 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature