Dataset Information

Destabilizing single chain major histocompatibility complex class I protein for repurposed enterokinase proteolysis.

ABSTRACT: The lack of a high throughput assay for screening stabilizing peptides prior to building a library of peptide-major histocompatibility complex class I (pMHC-I) molecules has motivated the continual use of in silico tools without biophysical characterization. Here, based on de novo protein fragmentation, the EASY MHC-I (EZ MHC-I) assay favors peptide antigen screening to an unheralded hands-on time of seconds per peptide due to the empty single chain MHC-I protein instability. Unlike tedious traditional labeling- and antibody-based MHC-I assays, repurposed enterokinase directly fragments the unstable single MHC-I chain protein unless rescued by a stabilizing peptide under luminal condition. Herein, the principle behind EZ MHC-I assay not only characterizes the overlooked stability as a known better indicator of immunogenicity than classical affinity but also the novel use of enterokinase from the duodenum to target destabilized MHC-I protein not bearing the standard Asp-Asp-Asp-Asp-Lys motif, which may protend to other protein instability-based assays.

SUBMITTER: Lim J

PROVIDER: S-EPMC7483518 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Destabilizing single chain major histocompatibility complex class I protein for repurposed enterokinase proteolysis.

Lim Jackwee J

Scientific reports 20200910 1

The lack of a high throughput assay for screening stabilizing peptides prior to building a library of peptide-major histocompatibility complex class I (pMHC-I) molecules has motivated the continual use of in silico tools without biophysical characterization. Here, based on de novo protein fragmentation, the EASY MHC-I (EZ MHC-I) assay favors peptide antigen screening to an unheralded hands-on time of seconds per peptide due to the empty single chain MHC-I protein instability. Unlike tedious trad ...[more]

PMID: 32913247

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Dataset Information

Destabilizing single chain major histocompatibility complex class I protein for repurposed enterokinase proteolysis.

Publications

Destabilizing single chain major histocompatibility complex class I protein for repurposed enterokinase proteolysis.

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