Molecular detection of Gram-positive bacteria in the human lung through an optical fiber-based endoscope.

ABSTRACT:

Purpose

The relentless rise in antimicrobial resistance is a major societal challenge and requires, as part of its solution, a better understanding of bacterial colonization and infection. To facilitate this, we developed a highly efficient no-wash red optical molecular imaging agent that enables the rapid, selective, and specific visualization of Gram-positive bacteria through a bespoke optical fiber-based delivery/imaging endoscopic device.

Methods

We rationally designed a no-wash, red, Gram-positive-specific molecular imaging agent (Merocy-Van) based on vancomycin and an environmental merocyanine dye. We demonstrated the specificity and utility of the imaging agent in escalating in vitro and ex vivo whole human lung models (n = 3), utilizing a bespoke fiber-based delivery

SUBMITTER: Mills B 

PROVIDER: S-EPMC7485201 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature