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Extensive and spatially variable within-cell-type heterogeneity across the basolateral amygdala.


ABSTRACT: The basolateral amygdala complex (BLA), extensively connected with both local amygdalar nuclei as well as long-range circuits, is involved in a diverse array of functional roles. Understanding the mechanisms of such functional diversity will be greatly informed by understanding the cell-type-specific landscape of the BLA. Here, beginning with single-cell RNA sequencing, we identified both discrete and graded continuous gene-expression differences within the mouse BLA. Via in situ hybridization, we next mapped this discrete transcriptomic heterogeneity onto a sharp spatial border between the basal and lateral amygdala nuclei, and identified continuous spatial gene-expression gradients within each of these regions. These discrete and continuous spatial transformations of transcriptomic cell-type identity were recapitulated by local morphology as well as long-range connectivity. Thus, BLA excitatory neurons are a highly heterogenous collection of neurons that spatially covary in molecular, cellular, and circuit properties. This heterogeneity likely drives pronounced spatial variation in BLA computation and function.

SUBMITTER: O'Leary TP 

PROVIDER: S-EPMC7486123 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Extensive and spatially variable within-cell-type heterogeneity across the basolateral amygdala.

O'Leary Timothy P TP   Sullivan Kaitlin E KE   Wang Lihua L   Clements Jody J   Lemire Andrew L AL   Cembrowski Mark S MS  

eLife 20200901


The basolateral amygdala complex (BLA), extensively connected with both local amygdalar nuclei as well as long-range circuits, is involved in a diverse array of functional roles. Understanding the mechanisms of such functional diversity will be greatly informed by understanding the cell-type-specific landscape of the BLA. Here, beginning with single-cell RNA sequencing, we identified both discrete and graded continuous gene-expression differences within the mouse BLA. Via in situ hybridization,  ...[more]

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