Characterization of CAR T cell expansion and cytotoxic potential during Ex Vivo manufacturing using image-based cytometry.

ABSTRACT: Since the FDA approval of two Chimeric Antigen Receptor (CAR) T cell therapies against CD19⁺ malignancies, there has been significant interest in adapting CAR technology to other diseases. As such, the ability to simultaneously monitor manufacturing criteria and functional characteristics of multiple CAR T cell products by a single instrument would likely accelerate the development of candidate therapies. Here, we demonstrate that image-based cytometry yields high-throughput measurements of CAR T cell proliferation and size, and captures the kinetics of in vitro antigen-specific CAR T cell-mediated killing. The data acquired and analyzed by the image cytometer are congruent with results derived from conventional technologies when tested contemporaneously. Moreover, the use of br

SUBMITTER: Maldini CR 

PROVIDER: S-EPMC7487036 | biostudies-literature | 2020 Sep - Oct

REPOSITORIES: biostudies-literature