Mutational resilience of antiviral restriction favors primate TRIM5α in host-virus evolutionary arms races.

ABSTRACT: Host antiviral proteins engage in evolutionary arms races with viruses, in which both sides rapidly evolve at interaction interfaces to gain or evade immune defense. For example, primate TRIM5α uses its rapidly evolving 'v1' loop to bind retroviral capsids, and single mutations in this loop can dramatically improve retroviral restriction. However, it is unknown whether such gains of viral restriction are rare, or if they incur loss of pre-existing function against other viruses. Using deep mutational scanning, we comprehensively measured how single mutations in the TRIM5α v1 loop affect restriction of divergent retroviruses. Unexpectedly, we found that the majority of mutations increase weak antiviral function. Moreover, most random mutations do not disrupt potent viral restriction, even w

SUBMITTER: Tenthorey JL 

PROVIDER: S-EPMC7492085 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature