Ontology highlight
ABSTRACT:
SUBMITTER: Collier JE
PROVIDER: S-EPMC7492086 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
eLife 20200915
In addition to extruding DNA loops, cohesin entraps within its SMC-kleisin ring (S-K) individual DNAs during G1 and sister DNAs during S-phase. All three activities require related hook-shaped proteins called Scc2 and Scc3. Using thiol-specific crosslinking we provide rigorous proof of entrapment activity in vitro. Scc2 alone promotes entrapment of DNAs in the E-S and E-K compartments, between ATP-bound engaged heads and the SMC hinge and associated kleisin, respectively. This does not require A ...[more]