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The measurement of the mediator and its influence on statistical mediation conclusions.

ABSTRACT: In psychology, the causal process between 2 variables can be studied with statistical mediation analysis. To make a causal interpretation about the relation between variables, researchers who use the statistical mediation model make many assumptions about the variables in the model, among which are measurement assumptions about the mediator. For example, researchers often assume that the measure of the mediator yields scores that are reliable and that have a valid interpretation. In this article, we address how several measurement challenges affect the conclusions of statistical mediation analysis, and how researchers can use different psychometric models to study theoretically different causal processes. We use simulated data sets to illustrate how 10 well-fitting and theoretically sound statistical mediation models could significantly detect the indirect effect or miss it entirely depending on how the mediator is represented in the model. In the example, power to detect the indirect effect varied by the amount of true mediator variance that the psychometric model of the mediator was able to isolate. Different strategies to incorporate psychometric methods into mediation research are discussed and future directions are considered. (PsycInfo Database Record (c) 2021 APA, all rights reserved).

SUBMITTER: Gonzalez O

PROVIDER: S-EPMC7492484 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Aims:It is generally accepted that post-MI heart failure (HF) changes a variety of aspects of sarcoplasmic reticular Ca2+ fluxes but for some aspects there is disagreement over whether there is an increase or decrease. The commonest statistical approach is to treat data collected from each cell as independent, even though they are really clustered with multiple likely similar cells from each heart. In this study, we test whether this statistical assumption of independence can lead the investigator to draw conclusions that would be considered erroneous if the analysis handled clustering with specific statistical techniques (hierarchical tests). Methods and results:Ca2+ transients were recorded in cells loaded with Fura-2AM and sparks were recorded in cells loaded with Fluo-4AM. Data were analysed twice, once with the common statistical approach (assumption of independence) and once with hierarchical statistical methodologies designed to allow for any clustering. The statistical tests found that there was significant hierarchical clustering. This caused the common statistical approach to underestimate the standard error and report artificially small P values. For example, this would have led to the erroneous conclusion that time to 50% peak transient amplitude was significantly prolonged in HF. Spark analysis showed clustering, both within each cell and also within each rat, for morphological variables. This means that a three-level hierarchical model is sometimes required for such measures. Standard statistical methodologies, if used instead, erroneously suggest that spark amplitude is significantly greater in HF and spark duration is reduced in HF. Conclusion:Ca2+ fluxes in isolated cardiomyocytes show so much clustering that the common statistical approach that assumes independence of each data point will frequently give the false appearance of statistically significant changes. Hierarchical statistical methodologies need a little more effort, but are necessary for reliable conclusions. We present cost-free simple tools for performing these analyses.

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The measurement of the mediator and its influence on statistical mediation conclusions.

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