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Penetration Process of a Hydrated Deep Eutectic Solvent Through the Stratum Corneum and its Application as a Protein Penetration Enhancer.

ABSTRACT: The penetration mechanism of choline chloride-glycerol deep eutectic solvent (DES) through the stratum corneum (SC) as a potential solvent for a novel enhancer of protein penetration into the skin was investigated in a wide and small angle X-ray diffraction study. We found that DES penetrated through intercellular lipids but not the corneocytes. DES seemed to extract a portion of lipids of the short lamellae in the SC. Hydrated DES with a DES to water weight ratio of 9 to 1 (9DES-1H2O) showed the strongest interaction with the lipids in the SC compared with water, DES, and hydrated DESs with another weight ratio of DES to water (DES?:?water=8?:?2). In a skin penetration test with a fluorescently labelled lysozyme, 9DES-1H2O increased the amount of penetration through the SC by two-fold compared with HEPES buffer.

SUBMITTER: Sakuragi M

PROVIDER: S-EPMC7493194 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Penetration Process of a Hydrated Deep Eutectic Solvent Through the Stratum Corneum and its Application as a Protein Penetration Enhancer.

Sakuragi Mina M Maeda Emika E Kusakabe Katsuki K

ChemistryOpen 20200916 9

The penetration mechanism of choline chloride-glycerol deep eutectic solvent (DES) through the stratum corneum (SC) as a potential solvent for a novel enhancer of protein penetration into the skin was investigated in a wide and small angle X-ray diffraction study. We found that DES penetrated through intercellular lipids but not the corneocytes. DES seemed to extract a portion of lipids of the short lamellae in the SC. Hydrated DES with a DES to water weight ratio of 9 to 1 (9DES-1H<sub>2</sub>O ...[more]

PMID: 32983826

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