IL-36? Promoted Wound Induced Hair Follicle Neogenesis via Hair Follicle Stem/Progenitor Cell Proliferation.

ABSTRACT: Background:Wound-induced hair follicle neogenesis (WIHN) is a phenomenon of hair neogenesis that occurs at the center of a scar when the wound area is sufficiently large. Neogenic hair follicles are separated from the pre-existing follicles at the wound edge by a hairless circular region. This WIHN study provides a unique model for developing treatments for hair loss and deciphering the mechanisms underlying organogenesis in adult mammals. Methods:The skin of a mouse was wounded by excising a 1.5 × 1.5 cm2 square of full-thickness dorsal skin. iTRAQ technology was used to screen proteins differentially expressed between the inner and outer scar areas in a mouse model of WIHN, on post-wounding day 15, to identify the regulators of WIHN. Owing to the overexpression of interleukin-36? (IL-36?) in the de novo hair follicle growth area, the regulating effect of IL-36? overexpression in WIHN was investigated. Hair follicle stem/progenitor cells were counted by flow cytometry while the expression of hair follicle stem/progenitor cell markers (Lgr5, Lgr6, Lrig1, K15, and CD34) and that of Wnt/?-catenin and IL-6/STAT3 pathway intermediaries was detected by qPCR and western blotting. Results:We found that wounding induced IL-36? expression. Incorporation of recombinant murine IL-36? (mrIL-36?) into murine skin wounds resulted in a greater number of regenerated hair follicles (p < 0.005) and a faster healing rate. The expression of hair follicle stem/progenitor cell markers was upregulated in the mrIL-36?-injected site (p < 0.05). Additionally, mrIL-36? upregulated the IL-6/STAT3 pathway intermediaries. Conclusion:IL-36? is upregulated in de novo hair follicle growth areas and can promote wound epithelialization and WIHN.

SUBMITTER: Gong L 

PROVIDER: S-EPMC7493638 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature