Dataset Information

Aging Disrupts Normal Time-of-Day Variation in Cardiac Electrophysiology.

ABSTRACT:

Background

Cardiac gene expression and arrhythmia occurrence have time-of-day variation; however, daily changes in cardiac electrophysiology, arrhythmia susceptibility, and Ca²⁺ handling have not been characterized. Furthermore, how these patterns change with age is unknown.

Methods

Hearts were isolated during the light (zeitgeber time [ZT] 4 and ZT9) and dark cycle (ZT14 and ZT21) from adult (12-18 weeks) male mice. Hearts from aged (18-20 months) male mice were isolated at ZT4 and ZT14. All hearts were Langendorff-perfused for optical mapping with voltage- and Ca²⁺-sensitive dyes (n=4-7/group). Cardiac gene and protein expression were assessed with real-time polymerase chain reaction (n=4-6/group) and Western blot (n=3-4/group).

Results

Adult hearts had the shortest action potential duration (APD) and Ca²⁺ transient duration (CaTD) at ZT14 (APD₈₀: ZT4: 45.4±4.1 ms; ZT9: 45.1±8.6 ms; ZT14: 34.7±4.2 ms; ZT21: 49.2±7.6 ms, P<0.05 versus ZT4 and ZT21; and CaTD₈₀: ZT4: 70.1±3.3 ms; ZT9: 72.7±2.7 ms; ZT14: 64.3±3.3 ms; ZT21: 74.4±1.2 ms, P<0.05 versus other time points). The pacing frequency at which CaT alternans emerged was faster, and average CaT alternans magnitude was significantly reduced at ZT14 compared with the other time points. There was a trend for decreased spontaneous premature ventricular complexes and pacing-induced ventricular arrhythmias at ZT14, and the hearts at ZT14 had diminished responses to isoproterenol compared with ZT4 (ZT4: 49.5.0±5.6% versus ZT14: 22.7±9.5% decrease in APD, P<0.01). In contrast, aged hearts exhibited no difference between ZT14 and ZT4 in nearly every parameter assessed (except APD₈₀: ZT4: 39.7±1.9 ms versus ZT14: 33.8±3.1 ms, P<0.01). Gene expression of KCNA5 (potassium voltage-gated channel subfamily A member 5; encoding Kv1.5) was increased, whereas gene expression of ADRB1 (encoding β1-adrenergic receptors) was decreased at ZT14 versus ZT4 in adult hearts. No time-of-day changes in expression or phosphorylation of Ca²⁺ handling proteins (SERCA2 [sarco/endoplasmic reticulum Ca²⁺-ATPase], RyR2 [ryanodine receptor 2], and PLB [phospholamban]) was found in ex vivo perfused adult isolated hearts.

Conclusions

Isolated adult hearts have strong time-of-day variation in cardiac electrophysiology, Ca²⁺ handling, and adrenergic responsiveness, which is disrupted with age.

SUBMITTER: Wang Z

PROVIDER: S-EPMC7494582 | biostudies-literature |

REPOSITORIES: biostudies-literature

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