Ontology highlight
ABSTRACT:
SUBMITTER: Zhou L
PROVIDER: S-EPMC7496532 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Angewandte Chemie (International ed. in English) 20200813 36
The non-enzymatic replication of the primordial genetic material is thought to have enabled the evolution of early forms of RNA-based life. However, the replication of oligonucleotides long enough to encode catalytic functions is problematic due to the low efficiency of template copying with mononucleotides. We show that template-directed ligation can assemble long RNAs from shorter oligonucleotides, which would be easier to replicate. The rate of ligation can be greatly enhanced by employing a ...[more]