Clinical Immunoassay for Human Hepcidin Predicts Iron Deficiency in First-Time Blood Donors.

ABSTRACT:

Background

Serum markers currently used as indicators of iron status have clinical limitations. Hepcidin, a key regulator of iron homeostasis, is reduced in iron deficiency (ID) and increased in iron overload. We describe the first CLIA-validated immunoassay with excellent accuracy and precision to quantify human serum hepcidin. Its diagnostic utility for detecting ID in first-time blood donors was demonstrated.

Methods

A monoclonal competitive ELISA (C-ELISA) was developed for the quantitation of human hepcidin and validated according to CLIA guidelines. Sera from nonanemic first-time blood donors (n = 292) were analyzed for hepcidin, ferritin, transferrin, and serum iron. Logistic regression served to determine the utility of hepcidin as a predictor of ID.

Results

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SUBMITTER: Gutschow P 

PROVIDER: S-EPMC7497288 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature