Robust estimation of bacterial cell count from optical density.
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ABSTRACT:
SUBMITTER: Beal J
PROVIDER: S-EPMC7499192 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Publications
Robust estimation of bacterial cell count from optical density.
Beal Jacob J Farny Natalie G NG Haddock-Angelli Traci T Selvarajah Vinoo V Baldwin Geoff S GS Buckley-Taylor Russell R Gershater Markus M Kiga Daisuke D Marken John J Sanchania Vishal V Sison Abigail A Workman Christopher T CT
Communications biology 20200917 1
Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to esti ...[more]
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