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VALERIE: Visual-based inspection of alternative splicing events at single-cell resolution.

ABSTRACT: We present VALERIE (Visualising alternative splicing events from single-cell ribonucleic acid-sequencing experiments), an R package for visualising alternative splicing events at single-cell resolution. To explore any given specified genomic region, corresponding to an alternative splicing event, VALERIE generates an ensemble of informative plots to visualise cell-to-cell heterogeneity of alternative splicing profiles across single cells and performs statistical tests to compare percent spliced-in (PSI) values across the user-defined groups of cells. Among the features available, VALERIE displays PSI values, in lieu of read coverage, which is more suitable for representing alternative splicing profiles for a large number of samples typically generated by single-cell RNA-sequencing experiments. VALERIE is available on the Comprehensive R Archive Network (CRAN): https://cran.r-project.org/web/packages/VALERIE/index.html.

SUBMITTER: Wen WX

PROVIDER: S-EPMC7500686 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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VALERIE: Visual-based inspection of alternative splicing events at single-cell resolution.

Wen Wei Xiong WX Mead Adam J AJ Thongjuea Supat S

PLoS computational biology 20200908 9

We present VALERIE (Visualising alternative splicing events from single-cell ribonucleic acid-sequencing experiments), an R package for visualising alternative splicing events at single-cell resolution. To explore any given specified genomic region, corresponding to an alternative splicing event, VALERIE generates an ensemble of informative plots to visualise cell-to-cell heterogeneity of alternative splicing profiles across single cells and performs statistical tests to compare percent spliced- ...[more]

PMID: 32898151

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