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In vivo Antidiabetic Activity Evaluation of Aqueous and 80% Methanolic Extracts of Leaves of Thymus schimperi (Lamiaceae) in Alloxan-induced Diabetic Mice.

ABSTRACT: Introduction:Diabetes mellitus disorder characterized by increase in serum glucose level as a result of change in fat, protein metabolism, and carbohydrate. The aim of the present study was to investigate the effects of the aqueous and hydroalcoholic leaf extract of Thymus. schimperi on blood glucose levels. Methods:The aqueous and 80% methanol extracts of T. schimperi leaves were prepared. Swiss albino mice of either sex weighing 20-30 g were selected for the experiments. Mice that were made diabetic were divided into seven groups to study the antihyperglycemic effect of the extracts. Diabetes was induced by single intraperitonial injection of alloxan monohydrate (180 mg/kg body weight). Results:After diabetic mice were treated with an extract of solvent at doses of 250 and 500 mg/kg for 21 days, there were significant decreases in fasting blood glucose when compared to diabetic controls. The observed antidiabetic activity could be associated with the phytochemicals present in this plant extract. The extract of solvent also prevented body weight loss of diabetic when compared to diabetic mice group. It was also observed that the extracts have shown no acute toxicity at a dose of 2 g/kg. Conclusion:The aqueous and 80% methanol extracts of T. schimperi leaves have shown blood glucose level lowering effects in diabetic mice. Hence, the present study might support the traditional use of T. schimperi for diabetes mellitus treatment.

SUBMITTER: Melesie Taye G

PROVIDER: S-EPMC7501980 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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In vivo Antidiabetic Activity Evaluation of Aqueous and 80% Methanolic Extracts of Leaves of <i>Thymus schimperi</i> (Lamiaceae) in Alloxan-induced Diabetic Mice.

Melesie Taye Getu G Bule Mohammed M Alemayehu Gadisa Diriba D Teka Frehiwot F Abula Teferra T

Diabetes, metabolic syndrome and obesity : targets and therapy 20200915

<h4>Introduction</h4>Diabetes mellitus disorder characterized by increase in serum glucose level as a result of change in fat, protein metabolism, and carbohydrate. The aim of the present study was to investigate the effects of the aqueous and hydroalcoholic leaf extract of <i>Thymus. schimperi</i> on blood glucose levels.<h4>Methods</h4>The aqueous and 80% methanol extracts of <i>T. schimperi</i> leaves were prepared. Swiss albino mice of either sex weighing 20-30 g were selected for the experi ...[more]

PMID: 32982351

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Project description:Diabetes mellitus is a chronic illness, and the management of diabetes is a global problem. Successful treatment is required to prevent complications and organ damages. Herbal medicines are having minimal adverse effects when compared to the available synthetic drugs to treat such chronic diseases and disorders.The present study was aimed to evaluate the antidiabetic and antioxidant activity of polar and nonpolar solvent extracts of Leucas aspera (Willd.) link leaves under in vitro models.The in vitro antidiabetic activity of petroleum ether (nonpolar) and ethanol (polar) extracts were evaluated in C2C12 cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (cell viability method) and glucose uptake assay. 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method used for the evaluation of in vitro antioxidant activity.Both the polar and nonpolar solvent extracts of L. aspera had shown better antioxidant activity compared to standard (IC50 = 18.96 and 19.90 μg/mL, respectively). Petroleum ether extract exhibited better cytotoxic activity in C2C12 cell line compared to ethanol extract (concentration of test drug needed to inhibit cell growth by 50% 110.75 ± 5.5 vs. 415.25 ± 8.0 μg/mL) whereas ethanol extract showed enhanced glucose uptake activity than petroleum ether extract in C2C12 cell line at same concentrations.From our study results, we concluded that L. aspera (Willd.) link leaves had shown better antidiabetic activity and antioxidant activity under in vitro models. Nonpolar solvent extract produced slightly better activity than polar solvent extract. This study warrants further research and experiments on animal models.Petroleum ether extract of Leucas aspera (PELA) exhibited slightly higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity compared to ethanol extract of L. aspera (EELA)PELA exhibited better cytotoxic activity in C2C12 cell line compared to EELAEELA had shown enhanced glucose uptake activity than PELA in C2C12 cell line at same concentrationsL. aspera leaf extracts have potential scavenging of DPPH radicals similar to that of ascorbic acidOverall, PELA (nonpolar) produced slightly better antidiabetic activity and antioxidant activity than EELA (polar). Abbreviations Used: DM: Diabetes mellitus, EELA: Ethanol extract of Leucas aspera, L. aspera: Leucas aspera, PELA: Petroleum ether extract of Leucas aspera.

Dataset Information

In vivo Antidiabetic Activity Evaluation of Aqueous and 80% Methanolic Extracts of Leaves of Thymus schimperi (Lamiaceae) in Alloxan-induced Diabetic Mice.

Publications

In vivo Antidiabetic Activity Evaluation of Aqueous and 80% Methanolic Extracts of Leaves of <i>Thymus schimperi</i> (Lamiaceae) in Alloxan-induced Diabetic Mice.

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