Dataset Information

Pairwise sequence comparison data of the DNA barcodes of aquatic insects.

ABSTRACT: This study compared the DNA sequences of cytochrome c oxidase subunit I (COI) and histone H3 of Ephemeroptera, Odonata, Plecoptera, and Trichoptera in a pairwise manner, and calculated the sequence similarities based on uncorrected P-distance (number of identical sites in both sequences per total number of the sites compared). Datasets of annotated sequences, the source organisms of which are identified at the species level in taxonomy, were retrieved from INSD (GenBank/ ENA/ DDBJ) as of the end of May 2020. Similarity scores of the pairwise comparison were sorted by the combinations of taxonomic groups; intraspecific variations, intrageneric-interspecific divergences, intrafamily-intergeneric divergences, and intraorder-interfamily divergences for Ephemeroptera, Odonata, Plecoptera, and Trichoptera. Similarity scores at the cumulative relative frequency points (1%, 5%, 10%, and median) may be used as the threshold to differentiate between the taxonomic groups based on sequence match. This is often done in the characterization of morphologically-unidentified specimens using barcode sequences, in the metabarcoding analysis of the local fauna, and environmental DNA analysis.

SUBMITTER: Inai K

PROVIDER: S-EPMC7502337 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Pairwise sequence comparison data of the DNA barcodes of aquatic insects.

Inai Koji K Wakimura Kei K Kato Mikio M

Data in brief 20200908

This study compared the DNA sequences of cytochrome c oxidase subunit I (COI) and histone H3 of Ephemeroptera, Odonata, Plecoptera, and Trichoptera in a pairwise manner, and calculated the sequence similarities based on uncorrected P-distance (number of identical sites in both sequences per total number of the sites compared). Datasets of annotated sequences, the source organisms of which are identified at the species level in taxonomy, were retrieved from INSD (GenBank/ ENA/ DDBJ) as of the end ...[more]

PMID: 32995390

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Project description:Recent estimates suggest that the global insect fauna includes fewer than six million species, but this projection is very uncertain because taxonomic work has been limited on some highly diverse groups. Validation of current estimates minimally requires the investigation of all lineages that are diverse enough to have a substantial impact on the final species count. This study represents a first step in this direction; it employs DNA barcoding to evaluate patterns of species richness in 27 orders of Canadian insects. The analysis of over one million specimens revealed species counts congruent with earlier results for most orders. However, Diptera and Hymenoptera were unexpectedly diverse, representing two-thirds of the 46 937 barcode index numbers (=species) detected. Correspondence checks between known species and barcoded taxa showed that sampling was incomplete, a result confirmed by extrapolations from the barcode results which suggest the occurrence of at least 94 000 species of insects in Canada, a near doubling from the prior estimate of 54 000 species. One dipteran family, the Cecidomyiidae, was extraordinarily diverse with an estimated 16 000 species, a 10-fold increase from its predicted diversity. If Canada possesses about 1% of the global fauna, as it does for known taxa, the results of this study suggest the presence of 10 million insect species with about 1.8 million of these taxa in the Cecidomyiidae. If so, the global species count for this fly family may exceed the combined total for all 142 beetle families. If extended to more geographical regions and to all hyperdiverse groups, DNA barcoding can rapidly resolve the current uncertainty surrounding a species count for the animal kingdom. A newly detailed understanding of species diversity may illuminate processes important in speciation, as suggested by the discovery that the most diverse insect lineages in Canada employ an unusual mode of reproduction, haplodiploidy.This article is part of the themed issue 'From DNA barcodes to biomes'.

Dataset Information

Pairwise sequence comparison data of the DNA barcodes of aquatic insects.

Publications

Pairwise sequence comparison data of the DNA barcodes of aquatic insects.

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