Dataset Information

LncRNA AC245100.4 binds HSP90 to promote the proliferation of prostate cancer.

ABSTRACT: Aim: To investigate the role and mechanisms of AC245100.4 in prostate cancer. Materials & methods: The expression and location of AC245100.4 were examined using real-time PCR and in situ hybridization. Cell Counting Kit-8, clone formation, flow cytometry and in vivo assays were conducted to determine the role of AC245100.4. RNA antisense purification with mass spectrometry and RNA immunoprecipitation were performed to identify proteins that bind to AC245100.4. Western blotting was performed to quantify the expression of protein. Results: AC245100.4 expression was upregulated in prostate cancer and mainly located in the cytoplasm. Knockdown of AC245100.4 inhibited proliferation of prostate cancer. Mechanistically, AC245100.4 bound to HSP90 and altered its chaperone function, increased the stability of IκB kinase and activated the NFκB signaling pathway. Conclusion: AC245100.4 promotes the proliferation of prostate cancer via binding of HSP90.

SUBMITTER: Cui R

PROVIDER: S-EPMC7506476 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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LncRNA AC245100.4 binds HSP90 to promote the proliferation of prostate cancer.

Cui Rongjun R Liu Chi C Lin Ping P Xie Hui H Wang Wei W Zhao Jiabin J Jiang Shan S Shi Jie J Yu Xiaoguang X

Epigenomics 20200819 15

Aim: To investigate the role and mechanisms of AC245100.4 in prostate cancer. Materials & methods: The expression and location of AC245100.4 were examined using real-time PCR and in situ hybridization. Cell Counting Kit-8, clone formation, flow cytometry and in vivo assays were conducted to determine the role of AC245100.4. RNA antisense purification with mass spectrometry and RNA immunoprecipitation were performed to identify proteins that bind t ...[more]

PMID: 32811161

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Dataset Information

LncRNA AC245100.4 binds HSP90 to promote the proliferation of prostate cancer.

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LncRNA <i>AC245100.4</i> binds HSP90 to promote the proliferation of prostate cancer.

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