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MAM domain containing 2 is a potential breast cancer biomarker that exhibits tumour-suppressive activity.

ABSTRACT: Objectives: The aim of this study was to discover new potential biomarkers of breast cancer and investigate their cellular functions.

Materials and methods: We analysed the gene expression profiles of matched pairs of breast tumour and normal tissues from 24 breast cancer patients. Tetracycline-inducible MAMDC2 expression system was established and used to evaluate cell proliferation in vitro and in vivo. MAMDC2-mediated signalling was determined using immunoblot analysis.

Results: We identified MAMDC2 as a down-regulated gene showing significant prognostic capability. Overexpression of MAMDC2 or treatment with MAMDC2-containing culture medium significantly inhibited the cell proliferation of T-47D cells. Furthermore, MAMDC2 expression reduced in vivo growth of T-47D xenograft tumours. MAMDC2 may exert its growth-inhibitory functions by attenuating the MAPK signalling pathway.

Conclusion: We report that MAMDC2 has a tumour-suppressive role and, as a secretory protein, it might be useful as a biomarker for breast cancer treatment.

SUBMITTER: Lee H

PROVIDER: S-EPMC7507446 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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MAM domain containing 2 is a potential breast cancer biomarker that exhibits tumour-suppressive activity.

Lee Hyeonhee H Park Bum-Chan BC Soon Kang Jong J Cheon Yeongmi Y Lee Soojin S Jae Maeng Pil P

Cell proliferation 20200724 9

<h4>Objectives</h4>The aim of this study was to discover new potential biomarkers of breast cancer and investigate their cellular functions.<h4>Materials and methods</h4>We analysed the gene expression profiles of matched pairs of breast tumour and normal tissues from 24 breast cancer patients. Tetracycline-inducible MAMDC2 expression system was established and used to evaluate cell proliferation in vitro and in vivo. MAMDC2-mediated signalling was determined using immunoblot analysis.<h4>Result ...[more]

PMID: 32707597

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MAM domain containing 2 is a potential breast cancer biomarker that exhibits tumour-suppressive activity.

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MAM domain containing 2 is a potential breast cancer biomarker that exhibits tumour-suppressive activity.

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