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Microflow-Based Device for In Vitro and Ex Vivo Drug Permeability Studies.

ABSTRACT: This paper presents a novel microflow-based concept for studying the permeability of in vitro cell models or ex vivo tissues. Using the proposed concept, we demonstrate how to maintain physiologically relevant test conditions and produce highly reproducible permeability values for a range (31) of drug compounds. The apparent permeability coefficients (Papp) showed excellent correlation (0.89) with the values from experiments performed with a conventional Ussing chamber. Additionally, the microflow-based concept produces notably more concentrated samples than the conventional Ussing chamber-based approach, despite the fact that more than 10 times smaller quantities of test compounds and biological membranes are needed in the microflow-based concept.

SUBMITTER: Hemmila S

PROVIDER: S-EPMC7509603 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Microflow-Based Device for In Vitro and Ex Vivo Drug Permeability Studies.

Hemmilä Samu S Ruponen Marika M Toropainen Elisa E Tengvall-Unadike Unni U Urtti Arto A Kallio Pasi P

SLAS technology 20200430 5

This paper presents a novel microflow-based concept for studying the permeability of in vitro cell models or ex vivo tissues. Using the proposed concept, we demonstrate how to maintain physiologically relevant test conditions and produce highly reproducible permeability values for a range (31) of drug compounds. The apparent permeability coefficients (Papp) showed excellent correlation (0.89) with the values from experiments performed with a conventional Ussing chamber. Additio ...[more]

PMID: 32351160

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