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Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2.

ABSTRACT: We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments.

SUBMITTER: Paden CR

PROVIDER: S-EPMC7510745 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2.

Paden Clinton R CR Tao Ying Y Queen Krista K Zhang Jing J Li Yan Y Uehara Anna A Tong Suxiang S

Emerging infectious diseases 20200701 10

We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments. ...[more]

PMID: 32610037

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