Detecting protein-protein interactions by Xe-129 NMR.
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ABSTRACT: Detection of protein-protein interactions (PPIs) is limited by current bioanalytical methods. A protein complementation assay (PCA), split TEM-1 β-lactamase, interacts with xenon at the interface of the TEM-1 fragments. Reconstitution of TEM-1-promoted here by cFos/cJun leucine zipper interaction-gives rise to sensitive 129Xe NMR signal in bacterial cells.
SUBMITTER: Zhao Z
PROVIDER: S-EPMC7511426 | biostudies-literature |
REPOSITORIES: biostudies-literature
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