Ontology highlight
ABSTRACT:
SUBMITTER: Smyrlaki I
PROVIDER: S-EPMC7511968 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Smyrlaki Ioanna I Ekman Martin M Lentini Antonio A Rufino de Sousa Nuno N Papanicolaou Natali N Vondracek Martin M Aarum Johan J Safari Hamzah H Muradrasoli Shaman S Rothfuchs Antonio Gigliotti AG Albert Jan J Högberg Björn B Reinius Björn B
Nature communications 20200923 1
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA in patient samples, but RNA extraction constitutes a major bottleneck in current testing. Methodological simplification could increase diagnostic availability and efficiency, benefitting patient care and infection control. Here, we describe methods circumventing RNA extraction in COVID-19 ...[more]