Ontology highlight
ABSTRACT:
SUBMITTER: Byrnes SA
PROVIDER: S-EPMC7927279 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Byrnes Samantha A SA Gallagher Ryan R Steadman Amy A Bennett Crissa C Rivera Rafael R Ortega Corrie C Motley S Timothy ST Jain Paras P Weigl Bernhard H BH Connelly John T JT
Analytical chemistry 20210225 9
The rapid onset of the global COVID-19 pandemic has led to challenges for accurately diagnosing the disease, including supply shortages for sample collection, preservation, and purification. Currently, most diagnostic tests require RNA extraction and detection by RT-PCR; however, extraction is expensive and time-consuming and requires technical expertise. With these challenges in mind, we report extraction-free, multiplexed amplification of SARS-CoV-2 RNA from 246 clinical samples, resulting in ...[more]