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Saliva Sampling and Its Direct Lysis, an Excellent Option To Increase the Number of SARS-CoV-2 Diagnostic Tests in Settings with Supply Shortages.


ABSTRACT: As part of any plan to lift or ease the confinement restrictions that are in place in many different countries, there is an urgent need to increase the capacity of laboratory testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Detection of the viral genome through reverse transcription-quantitative PCR (RT-qPCR) is the gold standard for this virus; however, the high demand of the materials and reagents needed to sample individuals, purify the viral RNA, and perform the RT-qPCR has resulted in a worldwide shortage of several of these supplies. Here, we show that directly lysed saliva samples can serve as a suitable source for viral RNA detection that is less expensive and can be as efficient as the classical protocol, which involves column purification of the viral RNA. In addition, it bypasses the need for swab sampling, decreases the risk of the health care personnel involved in the testing process, and accelerates the diagnostic procedure.

SUBMITTER: Moreno-Contreras J 

PROVIDER: S-EPMC7512180 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Saliva Sampling and Its Direct Lysis, an Excellent Option To Increase the Number of SARS-CoV-2 Diagnostic Tests in Settings with Supply Shortages.

Moreno-Contreras Joaquín J   Espinoza Marco A MA   Sandoval-Jaime Carlos C   Cantú-Cuevas Marco A MA   Barón-Olivares Héctor H   Ortiz-Orozco Oscar D OD   Muñoz-Rangel Asunción V AV   Hernández-de la Cruz Manuel M   Eroza-Osorio César M CM   Arias Carlos F CF   López Susana S  

Journal of clinical microbiology 20200922 10


As part of any plan to lift or ease the confinement restrictions that are in place in many different countries, there is an urgent need to increase the capacity of laboratory testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Detection of the viral genome through reverse transcription-quantitative PCR (RT-qPCR) is the gold standard for this virus; however, the high demand of the materials and reagents needed to sample individuals, purify the viral RNA, and perform the RT-q  ...[more]

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