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Hedgehog signaling is required for endomesodermal patterning and germ cell development in the sea anemone Nematostella vectensis.


ABSTRACT: Two distinct mechanisms for primordial germ cell (PGC) specification are observed within Bilatera: early determination by maternal factors or late induction by zygotic cues. Here we investigate the molecular basis for PGC specification in Nematostella, a representative pre-bilaterian animal where PGCs arise as paired endomesodermal cell clusters during early development. We first present evidence that the putative PGCs delaminate from the endomesoderm upon feeding, migrate into the gonad primordia, and mature into germ cells. We then show that the PGC clusters arise at the interface between hedgehog1 and patched domains in the developing mesenteries and use gene knockdown, knockout and inhibitor experiments to demonstrate that Hh signaling is required for both PGC specification and general endomesodermal patterning. These results provide evidence that the Nematostella germline is specified by inductive signals rather than maternal factors, and support the existence of zygotically-induced PGCs in the eumetazoan common ancestor.

SUBMITTER: Chen CY 

PROVIDER: S-EPMC7515634 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Hedgehog signaling is required for endomesodermal patterning and germ cell development in the sea anemone <i>Nematostella vectensis</i>.

Chen Cheng-Yi CY   McKinney Sean A SA   Ellington Lacey R LR   Gibson Matthew C MC  

eLife 20200924


Two distinct mechanisms for primordial germ cell (PGC) specification are observed within Bilatera: early determination by maternal factors or late induction by zygotic cues. Here we investigate the molecular basis for PGC specification in <i>Nematostella</i>, a representative pre-bilaterian animal where PGCs arise as paired endomesodermal cell clusters during early development. We first present evidence that the putative PGCs delaminate from the endomesoderm upon feeding, migrate into the gonad  ...[more]

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