Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mechanisms of tentacle morphogenesis in the sea anemone, Nematostella vectensis

ABSTRACT: Evolution of the capacity to form secondary outgrowths from the principal embryonic axes was a crucial innovation that potentiated the diversification of animal body plans. Nevertheless, precisely how such outgrowths develop in early-branching metazoan species remains poorly understood. To identify genes potentially involved in tentacle initiation, outgrowth, and maintenance, we performed transcriptional profiling at three different stages of Nematostella development: late planula larvae with tentacle buds, animals with growing tentacles, and mature animals with four-tentacle polyps. At each of these three stages, the animal was bisected such that the oral tentacle portion (head) could be compared to the body column.

ORGANISM(S): Nematostella vectensis

PROVIDER: GSE45588 | GEO | 2013/05/01

SECONDARY ACCESSION(S): PRJNA194626

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Mechanisms of tentacle morphogenesis in the sea anemone, Nematostella vectensis

Project description:Evolution of the capacity to form secondary outgrowths from the principal embryonic axes was a crucial innovation that potentiated the diversification of animal body plans. Nevertheless, precisely how such outgrowths develop in early-branching metazoan species remains poorly understood. To identify genes potentially involved in tentacle initiation, outgrowth, and maintenance, we performed transcriptional profiling at three different stages of Nematostella development: late planula larvae with tentacle buds, animals with growing tentacles, and mature animals with four-tentacle polyps. At each of these three stages, the animal was bisected such that the oral tentacle portion (head) could be compared to the body column. Tentacle buds, growing tentacles, and mature tentacles, were compared to body columns from each matching stage in duplicate, for a total of 6 samples.

2013-05-01 | E-GEOD-45588 | biostudies-arrayexpress

Geographical temperature stress in the sea anemone Nematostella vectensis

Project description:Gene expression of Nematostella vectensis from 5 different locations under chronic (30 day) temperature stress

2020-01-24 | GSE144104 | GEO

Nanos-2 mutant line of the sea anemone Nematostella vectensis

Project description:CRISPR/Cas9 mediated gene knockout show that nanos2+ progenitor cells are indispensable for male and female germline maintenance in Nematostella. This suggests nanos and piwi genes have a conserved role in somatic and germline stem cells in cnidarians.

2024-08-21 | GSE253068 | GEO

Graded FGF activity patterns distinct cell types within the apical sensory organ of the sea anemone Nematostella vectensis

Project description:Bilaterian animals have evolved complex sensory organs comprised of distinct cell types that function coordinately to sense the environment. Each sensory unit has a defined architecture built from component cell types, including sensory cells, non-sensory support cells, and dedicated sensory neurons. Whether this characteristic cellular composition is present in the sensory organs of non-bilaterian animals is unknown. Here, we interrogate the cell type composition and gene regulatory networks controlling development of the larval apical sensory organ in the sea anemone Nematostella vectensis. Using single cell RNA sequencing and imaging approaches, we reveal two unique cell types in the Nematostella apical sensory organ, GABAergic sensory cells and a putative non-sensory support cell population. Further, we identify the paired-like (PRD) homeodomain gene prd146 as a specific sensory cell marker and show that Prd146+ sensory cells become post-mitotic after gastrulation. Genetic loss of function approaches show that Prd146 is essential for apical sensory organ development. Using a candidate gene knockdown approach, we place prd146 downstream of FGF signaling in the apical sensory organ gene regulatory network. Further, we demonstrate that an aboral FGF activity gradient coordinately regulates the specification of both sensory and support cells. Collectively, these experiments define the genetic basis for apical sensory organ development in a non-bilaterian animal and reveal an unanticipated degree of complexity in a prototypic sensory structure.

2024-04-01 | GSE213278 | GEO

Molecular characterization of the apical organ in the anthozoan Nematostella vectensis

Project description:The aim of the experiment was the identification of genes that are expressed in the apical organ of the sea anemone Nematostella vectensis. To achieve this, animals were generated that either lack the apical organ (injected with a morpholino antisense oligonucleotide to block the function of NvFGFa1) or have an expanded apical organ (injected with a morpholino antisense oligonucleotide to block the function of NvFGFa2, see Rentzsch et al., Development, 2008). Eggs from the same batch were injected after fertilization, (MO NvFGFa1 at 0.5uM, MO NvFGFa2 at 0.25uM) and planula larvae with the expected phenotype and without general developmental abnormalities were selected after 72 hours of development at room temperature (22°C), and placed in RNAlater Solution (Ambion) for subsequent extraction of RNA. Three conditions were isolated: embryos injected with NvFGFa1 MO, embryos injected with NvFGFa2 MO and non-injected embryos. Total RNA was extracted with the RNAqueous kit (Ambion), following the manufacturer’s protocol, and temporarily stored at -80°C, in order to accumulate the quantity necessary for the hybridization. The isolated total RNA for each condition was pooled and split into to two tubes and shipped on dry ice to Nimblegen in Iceland. The injections and the isolation of total RNA were performed from May – August 2009 at the Sars Centre in Bergen, Norway.

2014-10-19 | E-MTAB-3004 | biostudies-arrayexpress

Nematostella vectensis

Project description:Nematostella vectensis RAD sequencing

| PRJNA170681 | ENA

Nematostella vectensis

Project description:Nematostella vectensis Genome sequencing

| PRJNA337090 | ENA

Post transcriptional modifications of miRNAs in the cnidarian Nematostella vectensis

Project description:MicroRNAs of bilaterian animals undergo posttranscriptional modifications such as methylation, tailing and trimming that regulate miRNA stability and function. To gain insight on the evolution of miRNA posttranscriptional modification, we studied regulation of miRNA stability by methylation in the sea anemone Nematostella vectensis, a representative of Cnidaria, the sister group of Bilateria.

2017-08-15 | GSE94526 | GEO

Nematostella vectensis

Project description:Nematostella vectensis Raw sequence reads

| PRJNA391807 | ENA

Nematostella vectensis

Project description:Nematostella vectensis Raw sequence reads

| PRJNA962884 | ENA