Dataset Information

Immune cell composition in normal human kidneys.

ABSTRACT: An understanding of immunological mechanisms in kidney diseases has advanced using mouse kidneys. However, the profiling of immune cell subsets in human kidneys remains undetermined, particularly compared with mouse kidneys. Normal human kidneys were obtained from radically nephrectomised patients with urogenital malignancy (n?=?15). Subsequently, human kidney immune cell subsets were analysed using multicolor flow cytometry and compared with subsets from C57BL/6 or BALB/c mice under specific pathogen-free conditions. Twenty kidney sections from healthy kidney donors or subjects without specific renal lesions were additionally analysed by immunohistochemistry. In human kidneys, 47%?±?12% (maximum 63%) of immune cells were CD3⁺ T cells. Kidney CD4⁺ and CD8⁺ T cells comprised 44% and 56% of total T cells. Of these, 47%?±?15% of T cells displayed an effector memory phenotype (CCR7^- CD45RA^- CD69^-), and 48%?±?19% were kidney-resident cells (CCR7^- CD45RA^- CD69⁺). However, the proportions of human CD14⁺ and CD16⁺ myeloid cells were approximately 10% of total immune cells. A predominance of CD3⁺ T cells and a low proportion of CD14⁺ or CD68⁺ myeloid cells were also identified in healthy human kidney sections. In mouse kidneys, kidney-resident macrophages (CD11b^low F4/80^high) were the most predominant subset (up to 50%) but the proportion of CD3⁺ T cells was less than 20%. These results will be of use in studies in which mouse results are translated into human cases under homeostatic conditions or with disease.

SUBMITTER: Park JG

PROVIDER: S-EPMC7515917 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Immune cell composition in normal human kidneys.

Park Jun-Gyu JG Na Myeongsu M Kim Min-Gang MG Park Su Hwan SH Lee Hack June HJ Kim Dong Ki DK Kwak Cheol C Kim Yon Su YS Chang Sunghoe S Moon Kyung Chul KC Lee Dong-Sup DS Han Seung Seok SS

Scientific reports 20200924 1

An understanding of immunological mechanisms in kidney diseases has advanced using mouse kidneys. However, the profiling of immune cell subsets in human kidneys remains undetermined, particularly compared with mouse kidneys. Normal human kidneys were obtained from radically nephrectomised patients with urogenital malignancy (n = 15). Subsequently, human kidney immune cell subsets were analysed using multicolor flow cytometry and compared with subsets from C57BL/6 or BALB/c mice under specific pa ...[more]

PMID: 32973321

Similar Datasets

Project description:Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related death in the world. Immunological analysis of the tumor microenvironment (immunoscore) shows great promise for improved prognosis and prediction of response to immunotherapy. However, the exact immune cell composition in NSCLC remains unclear. Here, we used flow cytometry to characterize the immune infiltrate in NSCLC tumors, non-cancerous lung tissue, regional lymph node, and blood. The cellular identity of >95% of all CD45+ immune cells was determined. Thirteen distinct immune cell types were identified in NSCLC tumors. T cells dominated the lung cancer landscape (on average 47% of all CD45+ immune cells). CD4+ T cells were the most abundant T cell population (26%), closely followed by CD8+ T cells (22%). Double negative CD4-CD8- T cells represented a small fraction (1.4%). CD19+ B cells were the second most common immune cell type in NSCLC tumors (16%), and four different B cell sub-populations were identified. Macrophages and natural killer (NK) cells composed 4.7 and 4.5% of the immune cell infiltrate, respectively. Three types of dendritic cells (DCs) were identified (plasmacytoid DCs, CD1c+ DCs, and CD141+ DCs) which together represented 2.1% of all immune cells. Among granulocytes, neutrophils were frequent (8.6%) with a high patient-to-patient variability, while mast cells (1.4%), basophils (0.4%), and eosinophils (0.3%) were less common. Across the cohort of patients, only B cells showed a significantly higher representation in NSCLC tumors compared to the distal lung. In contrast, the percentages of macrophages and NK cells were lower in tumors than in non-cancerous lung tissue. Furthermore, the fraction of macrophages with high HLA-DR expression levels was higher in NSCLC tumors relative to distal lung tissue. To make the method readily accessible, antibody panels and flow cytometry gating strategy used to identify the various immune cells are described in detail. This work should represent a useful resource for the immunomonitoring of patients with NSCLC.

Dataset Information

Immune cell composition in normal human kidneys.

Publications

Immune cell composition in normal human kidneys.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets