Ontology highlight
ABSTRACT:
SUBMITTER: Moro LN
PROVIDER: S-EPMC7518276 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Moro Lucia Natalia LN Viale Diego Luis DL Bastón Juan Ignacio JI Arnold Victoria V Suvá Mariana M Wiedenmann Elisabet E Olguín Martín M Miriuka Santiago S Vichera Gabriel G
Scientific reports 20200924 1
The application of new technologies for gene editing in horses may allow the generation of improved sportive individuals. Here, we aimed to knock out the myostatin gene (MSTN), a negative regulator of muscle mass development, using CRISPR/Cas9 and to generate edited embryos for the first time in horses. We nucleofected horse fetal fibroblasts with 1, 2 or 5 µg of 2 different gRNA/Cas9 plasmids targeting the first exon of MSTN. We observed that increasing plasmid concentrations improved mutation ...[more]