Generation of genome-edited dogs by somatic cell nuclear transfer.
Ontology highlight
ABSTRACT: Background
Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease.Results
We constructed a CRISPR-Cas9 vector targeting canine DJ-1. Genome-edited canine fibroblasts were established using vector transfection and antibiotic selection. We performed canine SCNT using genome-edited fibroblasts and successfully generated two genome-edited dogs. Both genome-edited dogs had insertion-deletion mutations at the target locus, and DJ-1 expression was either downregulated or completely repressed.Conclusion
SCNT successfully produced genome-edited dogs by using the CRISPR-Cas9 system for the first time.
SUBMITTER: Kim DE
PROVIDER: S-EPMC9281017 | biostudies-literature |
REPOSITORIES: biostudies-literature
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