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Effect of Aqueous Extract of Adansonia digitata Stem Bark on the Development of Hypertension in L-NAME-Induced Hypertensive Rat Model.

ABSTRACT: Background:Adansonia digitata is a plant used against cardiovascular disorders in African folk medicine. We assessed the effects of the aqueous extract of its stem bark on the development of hypertension in L-NAME-induced hypertensive rats. Methods:The animals were administered L-NAME once daily for 3 weeks (25?mg/kg, i.p.), concomitantly with aqueous extract of A. digitata stem bark (100 and 200?mg/kg, p.o.) or captopril (20?mg/kg, p.o.). Then, hemodynamic and electrocardiographic parameters, oxidative stress markers, and the lipid profile were assessed in the blood and heart, aorta, and kidney homogenates, and histopathological analyses were performed. Results:L-NAME-induced hypertensive control animals, but not the animals concomitantly treated with A. digitata extract, displayed increases in the mean arterial blood pressure (21.64% difference, p < 0.001, vs. dose 200?mg/kg), systolic arterial blood pressure (21.33%, p < 0.001), and the diastolic arterial blood pressure (21.84%, p < 0.001). In addition, hypertensive control animals displayed (i) increases in serum triglycerides, total cholesterol, LDL, and creatinine levels, malondialdehyde and transaminase activities, and atherogenic index; (ii) decreases in serum HDL, catalase, reduced glutathione, and nitric oxide; and (iii) aorta wall thickening, inflammatory cell infiltration, and cell loss in the cardiac muscle and renal tissues. As captopril, the extract prevented hypertension-like changes in lipid profile, cardiac, hepatic, and renal affection indicators, and oxidative stress markers. Conclusion:Our findings suggest that the extract of A. digitata has antihypertensive and antioxidant effects in L-NAME-induced hypertension rat models. These effects partly justify the traditional medicine use against cardiovascular disorders.

SUBMITTER: Ntchapda F

PROVIDER: S-EPMC7519996 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Effect of Aqueous Extract of <i>Adansonia digitata</i> Stem Bark on the Development of Hypertension in L-NAME-Induced Hypertensive Rat Model.

Ntchapda Fidèle F Bonabe Christian C Atsamo Albert Donatien AD Kemeta Azambou David Romain DR Bekono Fouda Yannick Y Imar Djibrine Soudy S Seke Etet Paul F PF Théophile Dimo D

Evidence-based complementary and alternative medicine : eCAM 20200918

<h4>Background</h4><i>Adansonia digitata</i> is a plant used against cardiovascular disorders in African folk medicine. We assessed the effects of the aqueous extract of its stem bark on the development of hypertension in L-NAME-induced hypertensive rats.<h4>Methods</h4>The animals were administered L-NAME once daily for 3 weeks (25 mg/kg, i.p.), concomitantly with aqueous extract of <i>A. digitata</i> stem bark (100 and 200 mg/kg, p.o.) or captopril (20 mg/kg, p.o.). Then, hemodynamic and elect ...[more]

PMID: 33014103

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Project description:Compared to other drug discovery sources, traditional medicine has significantly contributed to developing innovative therapeutic molecules for preventive and curative medicine. The Baobab tree, also known as Adansonia digitata L., is significant in Africa due to its multitude of benefits and various parts that serve different purposes, providing economic support to rural communities. The analysis of a plant sample using Fourier transform infrared (FT-IR) spectroscopy detected multiple functional groups, such as carboxyl and aromatic groups. Additionally, gas chromatography-mass spectroscopy (GC-MS) was utilized to identify various compounds present in the sample, including tetrachloroethylene and octyl ester. The results of different assays, such as α-diphenyl-β-picrylhydrazyl (DPPH), superoxide, nitric oxide scavenging assays, and total antioxidant by thiobarbituric acid method (TBA) and ferric thiocyanate (FTC) method, demonstrated a substantial scavenging of free radicals and an effective antioxidant efficacy. The bark's antimicrobial activity was tested through agar diffusion, resulting in a range of zone of inhibition from 10.1 ± 0.36 mm to 20.85 ± 0.76 mm. The minimum inhibitory concentration (MIC) value was observed to be approximately 0.625 µg/mL. The biofilm inhibition percentage ranged from 9.89% to 57.92%, with the highest percentage being 57.92%. The GC-MS and FT-IR studies revealed phytocompounds, which were then analyzed for their potential therapeutic properties. Computational studies were conducted on the phytocompounds against Pseudomonas aeruginosa and C2 kinase (antioxidant). The study concluded that the Adansonia digitata bark extract and its phytocompound have potential therapeutic efficacy against the target proteins. The best docking scores were about -7.053 kcal/mol and -7.573 kcal/mol for Pseudomonas aeruginosa and C2 kinase (antioxidant), respectively. The interaction patterns with the crucial amino acid residues elucidate the inhibitory efficacy of the phytocompounds.

Project description:Baobab (Adansonia digitata L) is a large tree species growing in semiarid and arid lowlands of Ethiopia and other places. The plant is valued by natives for its contributions as a cash crop and livelihood tree. Previous studies using samples from different countries have documented their phytochemical profiles and nutritional and health benefits. This study explored the phytochemical constituents and biological activities of fruit pulp extracts of baobab collected from Tekeze Valley, Tigrai, Ethiopia. To this end, qualitative phytochemical screening tests, quantitative phytochemical analyses, and gas chromatography-mass spectrometry (GC-MS) analysis were carried out using aqueous extract. Analyses of antioxidant activities were also conducted with aqueous- and methanol-extracts using of 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), and hydroxyl (OH) radical scavenging activity assays. The qualitative screening tests showed the presence of flavonoids, phenols, saponins, tannins, and terpenoids. Quantitative analyses of these phytochemicals at 25, 50, and 100 g/mL aqueous extract resulted in 0.0252 to 0.1000% yields. Yields of flavonoids, phenols, and saponins were higher at 50 g/mL extract, while that of tannins and terpenoids were higher at 100 g/mL. GC-MS analysis resulted in 15 predominant compounds including (1,2bis(trimethylsilyl)benzene (13.17%), 2-methyl-7-phenylindole (11.75%), 2-ethylacridine (10.11%), and benz[b]-1,4-oxazepine-4(5H)-thione,2,3-dihydro-2,8-dimethyl (10.11%). Aqueous and methanol extracts showed concentration-dependent antioxidant activities. In all the assays and concentrations, the antioxidant activities of both extracts were lower than that of the ascorbic acid standard. At equal extract concentrations (e.g., 100 and 250 μg/mL), methanol extract had higher antioxidant activities than aqueous extract. The findings can encourage future initiatives towards large-scale research for compiling a complete phytochemical profile of the fruit pulp of the Ethiopian baobab.

Dataset Information

Effect of Aqueous Extract of Adansonia digitata Stem Bark on the Development of Hypertension in L-NAME-Induced Hypertensive Rat Model.

Publications

Effect of Aqueous Extract of <i>Adansonia digitata</i> Stem Bark on the Development of Hypertension in L-NAME-Induced Hypertensive Rat Model.

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