Ontology highlight
ABSTRACT:
SUBMITTER: Thomas EN
PROVIDER: S-EPMC7521929 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Thomas Erica N EN Kim Kyusik Q KQ McHugh Emily P EP Marcinkiewicz Thomas T Zaher Hani S HS
eLife 20200917
Similar to DNA replication, translation of the genetic code by the ribosome is hypothesized to be exceptionally sensitive to small chemical changes to its template mRNA. Here we show that the addition of common alkylating agents to growing cultures of <i>Escherichia coli</i> leads to the accumulation of several adducts within RNA, including N(1)-methyladenosine (m<sup>1</sup>A). As expected, the introduction of m<sup>1</sup>A to model mRNAs was found to reduce the rate of peptide bond formation ...[more]