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Characterization of a New Transposon, Tn6696, on a bla NDM- 1-Carrying Plasmid From Multidrug-Resistant Enterobacter cloacae ssp. dissolvens in China.

ABSTRACT: Background:Enterobacter cloacae is an opportunistic pathogen which is responsible for serious nosocomial infections. A gene which plays an important role in resistance to carbapenems is the New Delhi metallo-?-lactamase 1 (NDM-1). Currently, the spread of NDM-1-producing E. cloacae strains is a serious public threat. Methods:A multidrug-resistant E. cloacae ssp. dissolvens strain CBG15936 was recovered in 2017 in Guangzhou, China. PCR, S1-pulsed-field gel electrophoresis, and Southern blotting were performed to locate the bla NDM - 1 gene. Susceptibility testing and conjugation experiments were also performed. Illumina HiSeq and Nanopore sequencers were used to perform whole-genome sequencing. Results:Strain CBG15936 belongs to ST932 and is resistant to carbapenems. The bla NDM- 1 gene was found on a ?62-kb plasmid, which has a conjugation frequency of 1.68 × 10-3 events per donor cell. Genome sequencing and analysis revealed that the NDM-1-carrying IncN1 plasmid contained a new transposon Tn6696, which consists of an intact qnrS1-carrying Tn6292 element, an inverted 8.3-kb Tn3000 remnant, ISkpn19, ?tnpA, and IS26. Conclusion:A new transposon, Tn6696, has been detected on a bla NDM- 1-carrying plasmid recovered from multidrug-resistant E. cloacae ssp. dissolvens CBG15936 from China. This finding provides a new perspective regarding the potential for bla NDM- 1 to undergo horizontal transfer among drug-resistant bacteria.

SUBMITTER: Chen Q

PROVIDER: S-EPMC7522282 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Characterization of a New Transposon, Tn6696, on a bla NDM- 1-Carrying Plasmid From Multidrug-Resistant Enterobacter cloacae ssp. dissolvens in China.

Chen Qichao Q Lin Yanfeng Y Li Zhonghong Z Lu Lanfen L Li Peihan P Wang Kaiying K Yang Lang L Ma Hui H Li Peng P Song Hongbin H

Frontiers in microbiology 20200915

<h4>Background</h4>Enterobacter cloacae is an opportunistic pathogen which is responsible for serious nosocomial infections. A gene which plays an important role in resistance to carbapenems is the New Delhi metallo-β-lactamase 1 (NDM-1). Currently, the spread of NDM-1-producing E. cloacae strains is a serious public threat.<h4>Methods</h4>A multidrug-resistant E. cloacae ssp. dissolvens strain CBG15936 was recovered in 2017 in Guangzhou, China. PCR, S1-pulsed-field g ...[more]

PMID: 33042048

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Dataset Information

Characterization of a New Transposon, Tn6696, on a bla NDM- 1-Carrying Plasmid From Multidrug-Resistant Enterobacter cloacae ssp. dissolvens in China.

Publications

Characterization of a New Transposon, Tn<i>6696</i>, on a <i>bla</i> <sub>NDM-</sub> <sub>1</sub>-Carrying Plasmid From Multidrug-Resistant <i>Enterobacter cloacae</i> ssp. <i>dissolvens</i> in China.

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