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Optimization of bio-chemical degumming of Ramie fiber for improved strength & luster.

ABSTRACT: Textile industries are currently not showing much interest in Ramie fibers due to the difficulties associated with their post-harvest downstream processing. The degumming chemicals are often detrimental to the environment upon discharged. Chemical degumming alone results in fibril-released coarse and brittle fibers. This problem has been addressed by combining partial chemical treatment with microbial degumming of the fibers for 72?h at 37?°C using a novel microbial formulation with Bacillus thuringiensis MCC2138 and Bacillus subtilis ABDR01. The extracellular microbial enzyme-based degumming without the release of fibrils produced a durable, soft, and lustrous fiber with higher tensile strength while utilizing fewer chemicals, thereby leading to lower discharge toxicity. The improved texture and strength compared to complete chemical treatment are attributed to even degumming of the fiber ensuring proper spinnability. Through this approach, Ramie is expected to gain visibility in the global textile market, thereby leading to Ramie cultivators' economic benefits.

SUBMITTER: Ray Chaudhuri S

PROVIDER: S-EPMC7528072 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Optimization of bio-chemical degumming of Ramie fiber for improved strength & luster.

Ray Chaudhuri Shaon S Gogoi Mandakini M Biswas Tethi T Chatterjee Soumya S Chanda Chaitali C Jamatia Ronald R Modak Ajoy A Sett Sunil K SK Mukherjee Indranil I

Biotechnology reports (Amsterdam, Netherlands) 20200922

Textile industries are currently not showing much interest in Ramie fibers due to the difficulties associated with their post-harvest downstream processing. The degumming chemicals are often detrimental to the environment upon discharged. Chemical degumming alone results in fibril-released coarse and brittle fibers. This problem has been addressed by combining partial chemical treatment with microbial degumming of the fibers for 72 h at 37 °C using a novel microbial formulation with <i>Bacillus ...[more]

PMID: 33024713

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Project description:BACKGROUND: The conventional degumming process of ramie with alkaline treatment at high temperature causes severe environmental pollution. Pectate lyases can be used to remove pectin from ramie in a degumming process with reduced environmental pollution and energy consumption. Pectate lyase PEL168 from Bacillus subtilis has been previously characterized and the protein structure was resolved. However, Bacillus is not a suitable host for pectate lyases during the degumming process since most Bacillus produce cellulases endogenously with a detrimental effect to the fiber. Pichia pastoris, which does not express endogenous cellulases and has high secretion capability, will be an ideal host for the expression. No previous work was reported concerning the heterologous expression of pectate lyase PEL168 in P. pastoris with an aim for industrial application in ramie bio-degumming. RESULTS: The gene pel168 was expressed in P. pastoris in this study. The recombinant protein PEL168 in P. pastoris (PEL168P) showed two bands of 48.6 kDa and 51.4 kDa on SDS-PAGE whereas the enzyme expressed in E. coli (PEL168E) was the same as predicted with a band of 46 kDa. Deglycosylation digestion suggested that PEL168P was glycosylated. The optimum reaction temperature of the two PEL168s was 50°C, and the optimum pH 9.5. After preincubation at 60°C for 20 min, PEL168E completely lost its activity, whereas PEL168P kept 26% of the residual activity. PEL168P had a specific activity of 1320 U/mg with a Km of 0.09 mg/ml and a Vmax of 18.13 ?mol/min. K?, Li?, Ni²? and Sr²? showed little or no inhibitory effect on PEL168P activity, and Ca²? enhanced enzyme activity by 38%. PEL168P can remove the pectin from ramie effectively in a degumming process. A 1.5 fold increase of PEL168 enzyme expression in P. pastoris was achieved by further codon optimization. CONCLUSIONS: Pectate lyase PEL168 with an available protein structure can be heterologously expressed in P. pastoris. The characterized recombinant PEL168P can be used to remove pectin from ramie efficiently and the expression level of PEL168 in P. pastoris was increased markedly by codon optimization. Therefore, PEL168 is an ideal candidate for further optimization and engineering for bio-degumming.

Dataset Information

Optimization of bio-chemical degumming of Ramie fiber for improved strength & luster.

Publications

Optimization of bio-chemical degumming of Ramie fiber for improved strength & luster.

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