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Cellular Delivery of Plasmid DNA into Wheat Microspores Using Rosette Nanotubes.

ABSTRACT: Plant genetic engineering offers promising solutions to the increasing demand for efficient, sustainable, and high-yielding crop production as well as changing environmental conditions. The main challenge for gene delivery in plants is the presence of a cell wall that limits the transportation of genes within the cells. Microspores are plant cells that are, under the right conditions, capable of generating embryos, leading to the formation of haploid plants. Here, we designed cationic and fluorescent rosette nanotubes (RNTs) that penetrate the cell walls of viable wheat microspores under mild conditions and in the absence of an external force. These nanomaterials can capture plasmid DNA to form RNT-DNA complexes and transport their DNA cargo into live microspores. The nanomaterials and the complexes formed were nontoxic to the microspores.

SUBMITTER: Cho JY

PROVIDER: S-EPMC7528298 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Cellular Delivery of Plasmid DNA into Wheat Microspores Using Rosette Nanotubes.

Cho Jae-Young JY Bhowmik Pankaj P Polowick Patricia L PL Dodard Sabine G SG El-Bakkari Mounir M Nowak Goska G Fenniri Hicham H Hemraz Usha D UD

ACS omega 20200916 38

Plant genetic engineering offers promising solutions to the increasing demand for efficient, sustainable, and high-yielding crop production as well as changing environmental conditions. The main challenge for gene delivery in plants is the presence of a cell wall that limits the transportation of genes within the cells. Microspores are plant cells that are, under the right conditions, capable of generating embryos, leading to the formation of haploid plants. Here, we designed cationic and fluore ...[more]

PMID: 33015458

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