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Coexistence of bla OXA-58 and tet(X) on a Novel Plasmid in Acinetobacter sp. From Pig in Shanghai, China.


ABSTRACT: The purpose of this study was to characterize the complete sequence of a novel plasmid carrying tigecycline resistance gene tet(X) and carbapenemase gene bla OXA-58 from a swine Acinetobacter sp. strain SH19PTT10. Minimal inhibitory concentration (MIC) was performed using microbroth dilution method. The isolate SH19PTT10 was highly resistant (16 mg/L) to tigecycline, and also exhibited resistance to ampicillin, streptomycin, tetracycline, chloramphenicol, florfenicol, ciprofloxacin, and sulfamethoxazole/trimethoprim. Although SH19PTT10 harbored bla OXA-58, it was susceptible to cefotaxime and meropenem. The genome sequence of SH19PTT10 was determined using PacBio single-molecule real-time sequencing. Plasmid pYUSHP10-1 had a size of 174,032 bp and showed partial homology to several plasmids found in Acinetobacter isolates. It contained two repA genes, putative toxin-antitoxin systems (HipA/HipB, RelE/RelB, and BrnT/BrnA), partitioning genes (parA and parB), and heavy metal resistance-associated genes (copA/copB, nrp, and czcA/czcD) but the transfer region or proteins was not found. pYUSHP10-1 carried 16 resistance genes, mainly clustered in two mosaic multiresistance regions (MRRs). The first MRR contained sul3, qacI-aadA1-clmA1-aadA2-blaCARB-2-dfrA16 cassette, aac(3)-IId, and bla OXA-58. The bla OXA-58 gene was associated with ISAba3, as previously described. The second MRR is the tet(X) region (ISAcsp12-aph(3')-Ia-IS26-?xerD-tet(X)-res-ISCR2-sul2) related to the corresponding region in other tet(X)-bearing plasmids. The pdif sites, as well as mobile elements, play an important role in mobilization of DNA modules and plasmid evolution. Coexistence of numerous resistance genes on a single plasmid may contribute to the dissemination of these genes under pressure posed by different agents, which may explain the presence of clinically crucial resistance genes tet(X) and bla OXA-58 in livestock. Thus, rational drug use and continued surveillance of tet(X) and bla OXA-58 in livestock are warranted.

SUBMITTER: Wang J 

PROVIDER: S-EPMC7530245 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Coexistence of <i>bla</i> <sub>OXA-58</sub> and <i>tet</i>(X) on a Novel Plasmid in <i>Acinetobacter</i> sp. From Pig in Shanghai, China.

Wang Jing J   Wang Yan Y   Wu Han H   Wang Zhen-Yu ZY   Shen Peng-Cheng PC   Tian Yu-Qi YQ   Sun Fan F   Pan Zhi-Ming ZM   Jiao Xinan X  

Frontiers in microbiology 20200918


The purpose of this study was to characterize the complete sequence of a novel plasmid carrying tigecycline resistance gene <i>tet</i>(X) and carbapenemase gene <i>bla</i> <sub>OXA-58</sub> from a swine <i>Acinetobacter</i> sp. strain SH19PTT10. Minimal inhibitory concentration (MIC) was performed using microbroth dilution method. The isolate SH19PTT10 was highly resistant (16 mg/L) to tigecycline, and also exhibited resistance to ampicillin, streptomycin, tetracycline, chloramphenicol, florfeni  ...[more]

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