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Mechanical stimulation of single cells by reversible host-guest interactions in 3D microscaffolds.

ABSTRACT: Many essential cellular processes are regulated by mechanical properties of their microenvironment. Here, we introduce stimuli-responsive composite scaffolds fabricated by three-dimensional (3D) laser lithography to simultaneously stretch large numbers of single cells in tailored 3D microenvironments. The key material is a stimuli-responsive photoresist containing cross-links formed by noncovalent, directional interactions between ?-cyclodextrin (host) and adamantane (guest). This allows reversible actuation under physiological conditions by application of soluble competitive guests. Cells adhering in these scaffolds build up initial traction forces of ~80 nN. After application of an equibiaxial stretch of up to 25%, cells remodel their actin cytoskeleton, double their traction forces, and equilibrate at a new dynamic set point within 30 min. When the stretch is released, traction forces gradually decrease until the initial set point is retrieved. Pharmacological inhibition or knockout of nonmuscle myosin 2A prevents these adjustments, suggesting that cellular tensional homeostasis strongly depends on functional myosin motors.

SUBMITTER: Hippler M

PROVIDER: S-EPMC7531888 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Mechanical stimulation of single cells by reversible host-guest interactions in 3D microscaffolds.

Hippler Marc M Weißenbruch Kai K Richler Kai K Lemma Enrico D ED Nakahata Masaki M Richter Benjamin B Barner-Kowollik Christopher C Takashima Yoshinori Y Harada Akira A Blasco Eva E Wegener Martin M Tanaka Motomu M Bastmeyer Martin M

Science advances 20200923 39

Many essential cellular processes are regulated by mechanical properties of their microenvironment. Here, we introduce stimuli-responsive composite scaffolds fabricated by three-dimensional (3D) laser lithography to simultaneously stretch large numbers of single cells in tailored 3D microenvironments. The key material is a stimuli-responsive photoresist containing cross-links formed by noncovalent, directional interactions between β-cyclodextrin (host) and adamantane (guest). This allows reversi ...[more]

PMID: 32967835

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Project description:Recently, chemical interface damping (CID) has been proposed as a new plasmon damping pathway based on interfacial hot-electron transfer from metal to adsorbate molecules. It has been considered essential, owing to its potential implications in efficient photochemical processes and sensing experiments. However, thus far, studies focusing on controlling CID in single gold nanoparticles have been very limited, and in situ reversible tuning has remained a considerable challenge. In these scanning electron microscopy-correlated dark-field spectroscopic measurements and density functional theory calculations, cucurbit[7]uril (CB[7])-based host-guest supramolecular interactions were employed to examine and control the CID process using monoamine-functionalized CB[7] (CB[7]-NH2) attached to single gold nanorods (AuNRs). In situ tuning of CID through the CB[7]-oxaliplatin complexation, which can result in the variation of the chemical nature and electronic properties of adsorbates, was presented. In addition, in situ tuning of CID was demonstrated through the competitive release of the oxaliplatin guest from the oxaliplatin@CB[7] complex, which was then replaced by a competitor guest of spermine in sufficient amounts. Furthermore, nuclear magnetic resonance experiments confirmed that the release of the guest is the consequence of adding salt (NaCl). Thus, in situ reversible tuning of CID in single AuNRs was achieved through successive steps of encapsulation and release of the guest on the same AuNR in a flow cell. Finally, single CB[7]-NH2@AuNRs were presented as a recyclable platform for CID investigations after the complete release of guest molecules from their host-guest inclusion complexes. Therefore, this study has paved a new route to achieve in situ reversible tuning of CID in the same AuNR and to investigate the CID process using CB-based host-guest chemistry with various guest molecules in single AuNRs for efficient hot-electron photochemistry and biosensing applications.

Dataset Information

Mechanical stimulation of single cells by reversible host-guest interactions in 3D microscaffolds.

Publications

Mechanical stimulation of single cells by reversible host-guest interactions in 3D microscaffolds.

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