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Mass spectrometric detection of nanoparticle host-guest interactions in cells.

ABSTRACT: Synthetic host-guest chemistry is a versatile tool for biomedical applications. Characterization and detection of host-guest complexes in biological systems, however, is challenging due to the complexity of the biological milieu. Here, we describe and apply a mass spectrometric method to monitor the association and dissociation of nanoparticle (NP)-based host-guest interactions that integrates NP-assisted laser desorption/ionization (LDI) and matrix assisted laser desoption/ionization (MALDI) mass spectrometry. This LDI/MALDI approach reveals how NP surface functionality affects host-guest interactions in cells, information difficult to achieve using other techniques.

SUBMITTER: Yan B

PROVIDER: S-EPMC4082387 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature

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Mass spectrometric detection of nanoparticle host-guest interactions in cells.

Yan Bo B Yesilbag Tonga Gulen G Hou Singyuk S Fedick Patrick W PW Yeh Yi-Cheun YC Alfonso Felix S FS Mizuhara Tsukasa T Vachet Richard W RW Rotello Vincent M VM

Analytical chemistry 20140610 13

Synthetic host-guest chemistry is a versatile tool for biomedical applications. Characterization and detection of host-guest complexes in biological systems, however, is challenging due to the complexity of the biological milieu. Here, we describe and apply a mass spectrometric method to monitor the association and dissociation of nanoparticle (NP)-based host-guest interactions that integrates NP-assisted laser desorption/ionization (LDI) and matrix assisted laser desoption/ionization (MALDI) ma ...[more]

PMID: 24873526

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