Ontology highlight
ABSTRACT:
SUBMITTER: Wang F
PROVIDER: S-EPMC7533693 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Wang Feng F Liu Yanxin Y Yu Zanlin Z Li Sam S Feng Shengjie S Cheng Yifan Y Agard David A DA
Proceedings of the National Academy of Sciences of the United States of America 20200910 39
Affinity grids have great potential to facilitate rapid preparation of even quite impure samples in single-particle cryo-electron microscopy (EM). Yet despite the promising advances of affinity grids over the past decades, no single strategy has demonstrated general utility. Here we chemically functionalize cryo-EM grids coated with mostly one or two layers of graphene oxide to facilitate affinity capture. The protein of interest is tagged using a system that rapidly forms a highly specific cova ...[more]