Ontology highlight
ABSTRACT:
SUBMITTER: Palovcak E
PROVIDER: S-EPMC6119484 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Journal of structural biology 20180711 1
Graphene oxide (GO) sheets have been used successfully as a supporting substrate film in several recent cryogenic electron-microscopy (cryo-EM) studies of challenging biological macromolecules. However, difficulties in preparing GO-covered holey carbon EM grids have limited their widespread use. Here, we report a simple and robust method for covering holey carbon EM grids with GO sheets and demonstrate that these grids can be used for high-resolution single particle cryo-EM. GO substrates adhere ...[more]