In situ structure of the Caulobacter crescentus flagellar motor and visualization of binding of a CheY-homolog.

ABSTRACT: Bacterial flagellar motility is controlled by the binding of CheY proteins to the cytoplasmic switch complex of the flagellar motor, resulting in changes in swimming speed or direction. Despite its importance for motor function, structural information about the interaction between effector proteins and the motor are scarce. To address this gap in knowledge, we used electron cryotomography and subtomogram averaging to visualize such interactions inside Caulobacter crescentus cells. In C. crescentus, several CheY homologs regulate motor function for different aspects of the bacterial lifestyle. We used subtomogram averaging to image binding of the CheY family protein CleD to the cytoplasmic Cring switch complex, the control center of the flagellar motor. This unambiguously confirmed the orie

SUBMITTER: Rossmann FM 

PROVIDER: S-EPMC7534056 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature