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SARS-CoV-2 envelope protein topology in eukaryotic membranes.

ABSTRACT: Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Ntlum/Ctcyt). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and host components and contribute to establish the basis to tackle the pathogenesis of SARS-CoV-2.

SUBMITTER: Duart G

PROVIDER: S-EPMC7536074 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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SARS-CoV-2 envelope protein topology in eukaryotic membranes.

Duart Gerard G García-Murria Mª Jesús MJ Grau Brayan B Acosta-Cáceres José M JM Martínez-Gil Luis L Mingarro Ismael I

Open biology 20200909 9

Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is ...[more]

PMID: 32898469

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