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Enhancing the expression of ARK1 genes in poplar leads to multiple branches and transcriptomic changes.

ABSTRACT: The ARBORKNOX1 (ARK1) gene is an important gene for regulating plant growth and development; however, transcriptomic responses of enhancing expression of ARK1 gene in poplar are poorly investigated. To provide insight into the gene function of the ARK1 gene in poplar, the ARK1 transgenic poplar '717' and '84 K' lines were obtained, the morphology of transgenic plants was observed, and transcriptome profiles were compared. The results showed that there were multiple branches in ARK1 transgenic seedlings compared with non-transgenic seedlings. The results of transcriptome analysis showed that there were significant differences in transcriptome profiles between the transgenic lines of '717' and '84 K', and between non-transgenic lines (CK) and transgenic plants. The real-time quantitative polymerase chain reaction (RT-qPCR) analysis confirmed the expression levels of the genes involved in the pathway of zeatin biosynthesis and brassinosteroid biosynthesis. The increase in expression levels of AHP and CYCD3 was related to multiple branches. Enhancing the expression of the ARK1 gene in poplar seedlings leads to multiple branches and transcriptomic changes.

SUBMITTER: Liu X

PROVIDER: S-EPMC7540752 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Enhancing the expression of ARK1 genes in poplar leads to multiple branches and transcriptomic changes.

Liu Xiaozhen X Zhang Zhiming Z Bian Wen W Duan Anan A Zhang Hanyao H

Royal Society open science 20200909 9

The ARBORKNOX1 (ARK1) gene is an important gene for regulating plant growth and development; however, transcriptomic responses of enhancing expression of ARK1 gene in poplar are poorly investigated. To provide insight into the gene function of the ARK1 gene in poplar, the ARK1 transgenic poplar '717' and '84 K' lines were obtained, the morphology of transgenic plants was observed, and transcriptome profiles were compared. The results showed that there were mult ...[more]

PMID: 33047064

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Enhancing the expression of ARK1 genes in poplar leads to multiple branches and transcriptomic changes.

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Enhancing the expression of <i>ARK1</i> genes in poplar leads to multiple branches and transcriptomic changes.

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