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CRISPRoff enables spatio-temporal control of CRISPR editing.

ABSTRACT: Following introduction of CRISPR-Cas9 components into a cell, genome editing occurs unabated until degradation of its component nucleic acids and proteins by cellular processes. This uncontrolled reaction can lead to unintended consequences including off-target editing and chromosomal translocations. To address this, we develop a method for light-induced degradation of sgRNA termed CRISPRoff. Here we show that light-induced inactivation of ribonucleoprotein attenuates genome editing within cells and allows for titratable levels of editing efficiency and spatial patterning via selective illumination.

SUBMITTER: Carlson-Stevermer J

PROVIDER: S-EPMC7542431 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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CRISPRoff enables spatio-temporal control of CRISPR editing.

Carlson-Stevermer Jared J Kelso Reed R Kadina Anastasia A Joshi Sahil S Rossi Nicholas N Walker John J Stoner Rich R Maures Travis T

Nature communications 20201007 1

Following introduction of CRISPR-Cas9 components into a cell, genome editing occurs unabated until degradation of its component nucleic acids and proteins by cellular processes. This uncontrolled reaction can lead to unintended consequences including off-target editing and chromosomal translocations. To address this, we develop a method for light-induced degradation of sgRNA termed CRISPRoff. Here we show that light-induced inactivation of ribonucleoprotein attenuates genome editing within cells ...[more]

PMID: 33028827

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