Ontology highlight
ABSTRACT:
SUBMITTER: Denervaud N
PROVIDER: S-EPMC3785771 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Dénervaud Nicolas N Becker Johannes J Delgado-Gonzalo Ricard R Damay Pascal P Rajkumar Arun S AS Unser Michael M Shore David D Naef Felix F Maerkl Sebastian J SJ
Proceedings of the National Academy of Sciences of the United States of America 20130909 39
Observing cellular responses to perturbations is central to generating and testing hypotheses in biology. We developed a massively parallel microchemostat array capable of growing and observing 1,152 yeast-GFP strains on the single-cell level with 20 min time resolution. We measured protein abundance and localization changes in 4,085 GFP-tagged strains in response to methyl methanesulfonate and analyzed 576 GFP strains in five additional conditions for a total of more than 10,000 unique experime ...[more]