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ABSTRACT:
SUBMITTER: Nishimura K
PROVIDER: S-EPMC7544234 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Nishimura Kohei K Yamada Ryotaro R Hagihara Shinya S Iwasaki Rie R Uchida Naoyuki N Kamura Takumi T Takahashi Koji K Torii Keiko U KU Fukagawa Tatsuo T
Nucleic acids research 20201001 18
The auxin-inducible degron (AID) system enables rapid depletion of target proteins within the cell by applying the natural auxin IAA. The AID system is useful for investigating the physiological functions of essential proteins; however, this system generally requires high dose of auxin to achieve effective depletion in vertebrate cells. Here, we describe a super-sensitive AID system that incorporates the synthetic auxin derivative 5-Ad-IAA and its high-affinity-binding partner OsTIR1F74A. The su ...[more]