Ontology highlight
ABSTRACT:
SUBMITTER: Ge MH
PROVIDER: S-EPMC7549056 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Ge Ming-Hai MH Wang Wei W Wu Tai-Hong TH Wen Xin X Al-Sheikh Umar U Chen Li-Li LL Yin Sheng-Wu SW Wu Jing-Jing JJ Huang Jia-Hao JH He Qing-Qin QQ Liu Hui H Li Rong R Wang Ping-Zhou PZ Wu Zheng-Xing ZX
iScience 20200915 10
Specific recording, labeling, and spatiotemporal manipulating neurons are essential for neuroscience research. In this study, we developed a tripartite spatiotemporal gene induction system in <i>C. elegans</i>, which is based on the knockout of two transcriptional terminators (stops in short) by two different recombinases FLP and CRE. The recombinase sites (<i>lox</i>P and <i>FRT</i>) flanked stops after a ubiquitous promoter terminate transcription of target genes. FLP and CRE, induced by two p ...[more]